Equipment Training Workshops

These workshops will be held at Cornell University to train and update participants in the use of the CIBT DNA and Protein Kits.  You may sign up for Saturday (DNA), Sunday (Protein), or both.  Participation qualifies you to borrow the related equipment from CIBT’s Equipment Lending Library.  Please note that each day can only accomodate 20 participants.

Breakfast, lunch, and in-service credit will be provided.

DNA Profiling

Saturday, January 19, 2008

In-Service Hours:  6

During this session, teachers will be introduced to the use of DNA in forensics.  The CIBT lab uses actual DNA and restriction enzymes.  Participants will do a restriction enzyme digestion of plasmid DNA, then separate the DNA fragments electrophoretically on an agarose gel.  The results simulate a DNA profile and demonstrate how this type of evidence is prepared and interpreted.

9:30 - 10:00	Check in, Breakfast
10:00 - 1:00	Lab in Comstock
1:00 - 1:30	Lunch
1:30 - 4:00	Lab
4:00 - 4:30	Wrap up, Evaluations

Protein Gel Electrophoresis

Sunday, January 20, 2008

In-Service Hours:  5

We will separate a mixture of proteins from a variety of meat samples (fishes, mammals, poultry and/or sea foods) using SDS-polyacrylamide gel electrophoresis (PAGE).  Evidence for evolutionary relatedness amongst organisms can be determined using this technique.  Participants will develop an experimental design, prepare samples of muscle tissue, observe the process of electrophoresis and analyze their results by comparing protein bands according to their molecular weights on the gel.

9:00 - 9:30	Registration, Breakfast
9:30 - 12:30	Lab in Comstock
12:30 - 1:00	Lunch
1:00 - 2:30	Lab in Comstock
2:30 - 3:00	Wrap up, Evaluations

Registration

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